Conformation Dynamics of DNA Molecules in Microchannels


This research was performed experimentally in hybridized microchannels made of PDMS and glass. Direct measurements have been conducted by hydrodynamic stretching DNA molecules in elongation flow via CLSM (Confocal Laser Scanning Microscope). The deformation of YOYO-labeled lambda phage DNA molecules in flows was visualized by CLSM (see Figure 1). Relations between DNA contour length and relaxation time in different models of stretching flows were also be presented and examined.
Furthermore, the evolution of stretching DNA molecules in microchannels was observed to analyze and compared with the DNA migration velocity and the corresponding stress modulus.