Conformation Dynamics of DNA Molecules in Microchannels – 微熱傳增強研究室

Conformation Dynamics of DNA Molecules in Microchannels

形變及動態特性研究-De

This research was performed experimentally in hybridized microchannels made of PDMS and glass. Direct measurements have been conducted by hydrodynamic stretching DNA molecules in elongation flow via CLSM (Confocal Laser Scanning Microscope). The deformation of YOYO-labeled lambda phage DNA molecules in flows was visualized by CLSM (see Figure 1). Relations between DNA contour length and relaxation time in different models of stretching flows were also be presented and examined.
Furthermore, the evolution of stretching DNA molecules in microchannels was observed to analyze and compared with the DNA migration velocity and the corresponding stress modulus.